Joe Mixon appeared in a Cincinnati courtroom Wednesday and pleaded not guilty to an aggravated menacing misdemeanor charge.
The Cincinnati Bengals running back is alleged to have "knowingly" pointed a gun in a woman's face, saying she "should be popped in the face."
The incident occurred less than a mile from the Bengals' stadium the same day the Bengals traveled to Buffalo to face the Bills in an AFC divisional round playoff game.
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A warrant was issued for Mixon's arrest in January, but the initial charge was dropped less than 24 hours after it was filed.
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However, police said new evidence prompted the filing of new charges.
"This decision was reached following the discovery of new evidence during the investigative process," the Cincinnati Police Department said in a statement Friday, via ESPN. "To preserve a fair and impartial judicial process for all parties involved, no investigatory details or evidence will be disclosed outside of official court proceedings."
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Mixon avoided charges from a shooting that occurred in his backyard that put a teenager in the hospital with non-life-threatening injuries. His sister and her boyfriend were both charged.